Introduction: Assess the needs of families is essential component to promote family centered care perception of parents’ need by nurses also has effective importance This study was designed to meet the needs of mothers admitted to neonatal intensive care unit.  
Method: This study is a descriptive analytic study sample size based on pilot study was 96 mothers.  In this study data were collected by using demographic data of mothers, fathers and infants check list and a 56-item questionnaire CCFNI that has 5 subscale sub- support, comfort, information, proximity, and confidence. The data were analyzed by using Independent t-test, Pearson correlation coefficient.
Results: The findings of the study showed that the mean (± SD) age of the mothers 27/87 (6/01 ±). The most important need “to have questions answered honestly about neonatal “, and the least important was “Have friends nearby for support “generally based on perception of mothers assurance needs was the most important subscale Confidence whit mean (± SD) 3/79 (0/22) and supportive needs was the least important subscale 3/28 (0/38).
Conclusion: Base of the result assurance of health and welfare of child are very important to mothers, in charge of NICU have to give sufficient information regarding neonate situation and the treatments, in this was assurance comes to mothers and lower stress of mothers in duration of hospitalization of neonate.