Introduction: evidence-based nursing guidelines are the gold standard in increasing quality of clinical implementation and nurses' knowledge. Neonatal Intensive Care Unit nurses to provide high quality of care, need to have a valid guidelines, could provide appropriate services to high-risk infants. The most important factor in improving the quality of care is evaluation of nurses' knowledge and awareness.The aim of this study was “Effect of education of evidence-based nursing guidelines on the nurses' knowledge in the NICU”.
Methods: This intervention study is done in, all nurses working in the NICU Baqiyatallah (AJ) hospital in Tehran city in 2014. Before and after of guidelines education, nurses' knowledge was measured by tests accordance to twenty guidelines.
 Results: The mean scores before education was 40(6.03), two weeks after education75.41 (8.10) and two months after education 95.83(6.33). Significant statistical difference via between the mean scores before and after education in the intervention group compared with the control group. (P=0.0001).
 Conclusion: According to effective role of the evidence-based guidelines as a valuable standard in promoting quality health care services, increase nurses' knowledge and raising the level of public health therefore it is recommended to the authorities and managers of the hospitals have coherent planning for education of evidence-based nursing guidelines through workshops and educational meetings.